[Observation the electroporation of blood cells in vitro by scanning electron microscope].

OBJECTIVE To observe changes on cell membrane in blood cells after they were been electrified. METHODS Blood were electrified for 5, 10, 20, 30 s, 1 min respectively, and Scanning electron microscope was used to detect the changes on their cell membranes. RESULTS Pores were detected both on electrified erythrocytes and leukocytes with round or ellipse shapes. The erythrocytes often have one or more pores while the leukocytes often have more pores looked like cribble. The rates of perforated cells were increased with the prolonging time of electrification, 5 s with 6% and 1 min increased to 40%. CONCLUSIONS Alternating current can cause the cell perforating, and the rates of perforated cell were increased with the prolonging time of electrification.